IL6基因内含子3多态性与仔猪腹泻、生长和公猪繁殖性状的关联分析

doi:10.11843/j.issn.0366-6964.2017.09.012

摘要/Abstract

摘要：

旨在研究猪IL6基因的多态性及其与猪经济性状相关的关联性，寻找与仔猪腹泻、生长和公猪繁殖性状相关的分子标记。利用PCR扩增在IL6基因的内含子3，检测到1个SNP位点g.1704674C>T，该SNP可引起Hpa Ⅱ酶切位点的改变。通过PCR-RFLP检测281头民猪仔猪、182头长白仔猪、215头杜洛克公猪以及69头大白公猪IL6基因的多态性，分析多态位点与仔猪腹泻、生长和公猪繁殖性状的关联性；利用Real-time PCR技术分析IL6基因在LPS刺激民猪外周血单个核细胞（Peripheral blood mononuclear cell，PBMC）后的表达模式。结果表明，在民猪仔猪中，CC型个体的腹泻指数显著高于TT型个体（P<0.05），CC型个体的14、21、28、35日龄体重及日增重均显著低于TC型个体（P<0.05），且CC型个体的21日龄体重也显著低于TT型个体（P<0.05）；在长白仔猪中，TT型个体的出生重显著高于CC型个体（P<0.05），7日龄体重显著高于TC型个体（P<0.05）；在杜洛克公猪中，TT和TC基因型个体射精量显著大于CC基因型个体（P<0.05）；在大白公猪中，CC基因型个体精子密度显著大于TC基因型个体（P<0.05）。PBMC受LPS刺激12和24 h后，IL6基因表达量均显著升高（P<0.01或P<0.05）。综上表明，IL6基因g.1704674C>T位点对仔猪腹泻、生长以及公猪繁殖都有一定的影响，可作为猪育种过程中一个潜在的分子标记。

Abstract:

In order to discover new molecular marker related to piglet diarrhea, growth and boar reproduction traits, the polymorphism of porcine IL6 gene was investigated and the association analysis was performed in different pig breeds. The DNA fragment of IL6 gene was amplified and sequenced. A SNP (g.1704674C>T), which could alter the recognition site of HpaⅡ, was detected in intron 3 of IL6. The SNP was genotyped by PCR-RFLP in 281 Min piglets, 182 Landrace piglets, 215 Duroc boars and 69 Yorkshire boars. Its association with piglet diarrhea, growth and boar reproduction traits was analyzed. The expression of IL6 was detected in PBMC stimulated with LPS by real-time PCR. The results showed that Min piglets with CC genotype had significantly higher diarrhea index than TT genotype piglets (P<0.05). The 14, 21, 28, 35-day body weight and ADG of Min piglets with CC genotype were significantly lower than that of TC genotype (P<0.05), and the 21-day body weight of Min piglets was also significantly lower than that of piglets with TT genotype (P<0.05). In Landrace piglets, birth weight of piglets with TT genotype was significantly higher than that of piglets with CC genotype (P<0.05), and the 7-day body weight was significantly higher than that of individuals with TC genotype (P<0.05). In Duroc boars, the ejaculate volume of boars with TT and TC genotype was significantly higher than that of boars with CC genotype (P<0.05). In Yorkshire boar, the sperm density of boars with CC genotype was significantly higher than that of boars with TC genotype (P<0.05). At 12 and 24 h after LPS stimulation, the expression of IL6 gene was significantly increased in PBMC (P<0.01 or P<0.05). Therefore, it can be concluded IL6 g.1704674C> T had effect on piglet diarrhea, growth and boar reproduction traits, and which may be a potential molecular marker in pig breeding.

中图分类号:

S813.1

查安东, 邢桂玲, 高晓雯, 张龙, 狄生伟, 王希彪, 牛步月. IL6基因内含子3多态性与仔猪腹泻、生长和公猪繁殖性状的关联分析[J]. 畜牧兽医学报, 2017, 48(9): 1674-1682.

ZHA An-dong, XING Gui-ling, GAO Xiao-wen, ZHANG Long, DI Sheng-wei, WANG Xi-biao, NIU Bu-yue. Association Analysis between IL6 Gene Intron 3 Polymorphism and Piglet Diarrhea, Growth and Boar Reproduction Traits[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(9): 1674-1682.

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